This study builds on current studies on this area by examining job satisfaction and employee’s turnover in mission hospitals in Bono Region. Stratified and simple random sampling techniques were used to select 193 and 198 respondents from Holy Family Hospital-Berekum and Methodist Hospital-Wenchi respectively. Data were analyzed using descriptive statistics, correlation and logistic regression. Findings revealed that poor remuneration package is a prevailing cause of employees’ turnover at mission hospitals in Bono Region. Findings also revealed that there is inverse relation and effect of job satisfaction and employee’s turnover at the mission hospitals in Bono region. Findings also revealed that there exist employees’ turnover challenges at the mission hospitals. The study recommends that more effort should be made to look at the variables, leading to the construct of underlying causes of employees’ turnover, such as poor working environment, poor remuneration packages, employees and management relationship and lack of career progression, human resource departments should improve measures to address employees’ turnover intension to mitigate challenges posed by turnover at the mission hospitals. It also recommends that further studies on job satisfaction and turnover at mission hospitals should include other regions.